badionotus, as well as on other types of ocean cucumbers, look in these rooked variety for the Colombia could have been restricted to a few degree towards the wealth and you may shipping [dos, 16] and you may reproductive biology [17, 18]. But not, you may still find of several gaps when you look at the training, eg regarding their reproductive models as well as how he’s influenced by variables instance temperature, salinity, rain, and you will restaurants supply.
The current studies refers to aspects of brand new reproductive biology of holothuroids We. badionotus and you will Isostichopus sp. about Santa Marta Region utilising the gonadal list (GI) and you can histological study of new gonads. This study is meant to enhance the newest understanding of the biology and you may ecology out of sea cucumbers native to the fresh new Colombian Caribbean Water and offer a scientific cause for the introduction of management procedures and you will maintenance of the capital.
dos. Material and techniques
Specimens out-of Isostichopus sp. had been amassed of , when you are specimens away from Isostichopus badionotus had been compiled from from the Taganga Bay (11° 16′ ” N– 74° 11′ ” W) and Rodadero Bay (11° 12′ ” N– 74° 13′ ” W) on Caribbean region of Santa Marta, Colombia (Fig step 1).
It also assesses brand new determine regarding environment and chemical substances variables (temperatures, rain, the percentage of normal carbon dioxide articles regarding the deposit) on the reproductive biology and you may assesses the dwelling of their populations (frequency out-of duration and you will lbs, sex proportion, mean dimensions and you can weight initially sexual maturity, fecundity)
Package from inside the inset A great try magnified for the inset B; container during the inset B is actually magnified in the primary panel. Source: Recommendations legs INVEMAR.
As a result of the wide variety of molds and colours which i. badionotus enjoys, this study concerned about the newest collection of bacteria most abundant in common morphotype of types found in the research area, explained from the Vergara et al. as i. badionotus morphotype II.
Monthly fourteen to 20 folks of each morphotype (I. badionotus and you can Isostichopus sp.) had been gathered by snorkeling. For this function, a pair of snorkelers swam a good transect 5 yards large by whenever 1000 m long, double. The first travels was developed bordering the newest coast range therefore the next well away of approximately ten m on coast range. The most browse depth was ten meters to possess logistical and you can security reasons and although it’s likely that discover some one at greater distance and you may depth, this might never be reviewed in today’s data. During the October, no choices will be made considering the passing of Hurricane Matthew, and therefore inspired the brand new Colombian Caribbean during that few days. The ocean cucumbers have been individually manufactured during the plastic handbags having seawater and directed on the Aquaculture Laboratory from Universidad del Magdalena. The breadth regarding just take of any private therefore the variety of bottom they occupied was indeed filed (categorized just like the rocky, sandy, red coral, rock-red coral, rock-mud, rock-sand-coral). On top of that, body sediment trials (having a density not greater than 10 mm) was basically taken close to the individuals dictate new part of all-natural carbon dioxide content playing with a bulk spectrometer, Euro EA 3000 best hookup apps Scottsdale elemental analyzer (EuroVector). Salinity and you can drinking water temperature was in fact submitted monthly, if you’re complete rain investigation was basically obtained from the fresh new info of your Simon Bolivar airport meteorological station of town of Santa Marta.
People have been forfeited regarding the laboratory by position them from inside the seawater during the cuatro°C, which had been along with meant to arrived at a max condition of contraction one allowed to reduce the mistake throughout the size dimensions, because of the potential that they need to expand or bargain in the commonly. Then following body measurements have been removed: full looks duration (TL), having fun with a flexible step one-m tape scale at the nearby 0.5 cm (shortly after compromise because of the thermal amaze new contracted system had a c otherwise S shape, in addition to tape contoured your body, so that the complete length ‘s the contour length); gonad regularity playing with a finished cylinder during the nearest 0.1 cm 3 ; system wall surface pounds (BW), recognized as the weight of the individual without viscera otherwise gonad and gonad lbs (GW) in the nearest 0.1 g. Gonad list (GI) is actually determined making use of the formula, GI = (GW/ BW)* one hundred [19, 20].
